Direct whole genome sequencing of sputum accurately identifies drug resistant Mycobacterium tuberculosis faster than MGIT culture sequencing (Record no. 76655)
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| 000 -LEADER | |
|---|---|
| fixed length control field | 03170cam a2200157 4500 |
| 001 - CONTROL NUMBER | |
| control field | NMDX7915 |
| 008 - FIXED-LENGTH DATA ELEMENTS--GENERAL INFORMATION | |
| fixed length control field | 120401t2018 xxu||||| |||| 00| 0 eng d |
| 100 ## - MAIN ENTRY--PERSONAL NAME | |
| Personal name | Lozewicz, S. |
| 240 ## - UNIFORM TITLE | |
| Uniform title | <a href="Journal of Clinical Microbiology">Journal of Clinical Microbiology</a> |
| 245 ## - TITLE STATEMENT | |
| Title | Direct whole genome sequencing of sputum accurately identifies drug resistant Mycobacterium tuberculosis faster than MGIT culture sequencing |
| 260 ## - PUBLICATION, DISTRIBUTION, ETC. (IMPRINT) | |
| Date of publication, distribution, etc. | 2018 |
| 500 ## - GENERAL NOTE | |
| General note | NMUH Staff Publications |
| 520 ## - SUMMARY, ETC. | |
| Summary, etc. | <span style="font-size: 10pt;"><span style="font-family: arial, helvetica, clean, sans-serif; text-align: left;">The current methods available to diagnose antimicrobial resistant&nbsp;</span><em style="font-family: arial, helvetica, clean, sans-serif; text-align: left;">Mycobacterium tuberculosis</em><span style="font-family: arial, helvetica, clean, sans-serif; text-align: left;">&nbsp;infections require positive culture or only test a limited number of resistance-associated mutations. Rapid, accurate identification of antimicrobial resistance enables prompt initiation of effective treatment. Here, we determine the utility of whole-genome sequencing (WGS)&nbsp;</span><em style="font-family: arial, helvetica, clean, sans-serif; text-align: left;">M. tuberculosis</em><span style="font-family: arial, helvetica, clean, sans-serif; text-align: left;">&nbsp;directly from routinely obtained diagnostic sputum samples to provide a comprehensive resistance profile compared to Mycobacterial Growth Indicator Tube (MGIT) WGS. We sequenced&nbsp;</span><em style="font-family: arial, helvetica, clean, sans-serif; text-align: left;">M. tuberculosis</em><span style="font-family: arial, helvetica, clean, sans-serif; text-align: left;">&nbsp;from 43 sputum samples by targeted DNA enrichment using the Agilent SureSelectXT kit, and 43 MGIT positive samples from each participant. Thirty two (74%) sputum samples and 43 (100%) MGIT samples generated whole genomes. Time to antimicrobial resistance profile and concordance was compared with Xpert MTB/RIF and phenotypic resistance testing from culture of the same samples. Antibiotic susceptibility could be predicted from WGS of sputum within 5 days of sample receipt and up to 24 days earlier than WGS from MGIT culture and up to 31 days earlier than phenotypic testing. Direct sputum results could be reduced to 3 days with faster hybridisation and if only regions encoding drug resistance are sequenced. We show that direct sputum sequencing has the potential to provide comprehensive resistance detection significantly faster than MGIT whole genome sequencing or phenotypic testing of resistance from culture in a clinical setting. This improved turnaround time enables prompt, appropriate treatment with associated patient and health service benefit. Improvements in sample preparation are necessary to ensure comparable sensitivity and complete resistance profile predictions in all cases.</span>&nbsp;</span> |
| 856 ## - ELECTRONIC LOCATION AND ACCESS | |
| Uniform Resource Identifier | <a href="https://www.ncbi.nlm.nih.gov/pubmed/29848567">https://www.ncbi.nlm.nih.gov/pubmed/29848567</a> |
| 856 ## - ELECTRONIC LOCATION AND ACCESS | |
| Uniform Resource Identifier | <a href="http://jcm.asm.org/content/early/2018/05/24/JCM.00666-18.long">http://jcm.asm.org/content/early/2018/05/24/JCM.00666-18.long</a> |
| Withdrawn status | Lost status | Damaged status | Not for loan | Collection code | Home library | Current library | Shelving location | Date acquired | Total Checkouts | Date last seen | Price effective from | Koha item type |
|---|---|---|---|---|---|---|---|---|---|---|---|---|
| Staff publications for NMDX | Ferriman information and Library Service (North Middlesex) | Ferriman information and Library Service (North Middlesex) | Shelves | 07/06/2022 | 07/06/2022 | 07/06/2022 | Book |